dapi based vectashield Search Results


4 6 diamidino 2 phenylindole  (Vector Laboratories)
98
Vector Laboratories 4 6 diamidino 2 phenylindole
CBA to detect anti-MuSK Abs. When HEK293 cells expressing MuSK were incubated with (A) commercially available polyclonal anti-MuSK Abs or (B) sera from patients with MuSK-Ab-positive MG, anti-IgG antibody binding (red fluorescence) was observed along the cell surface, indicating the binding of MuSK Abs to MuSK. This colocalized with GFP-labeled MuSK expressed on the surface of HEK293 cells (green fluorescence). In contrast, (C) anti-human IgG antibody binding (red fluorescence) was not observed after adding the sera from the healthy control. Scale bar: 10 µm. CBA: cell-based assay, <t>DAPI:</t> <t>4′,6-diamidino-2-phenylindole,</t> eGFP: enhanced green fluorescent protein, HEK293: human embryonic kidney 293, MG: myasthenia gravis, MuSK Abs: antibodies against muscle-specific tyrosine kinase.
4 6 Diamidino 2 Phenylindole, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4 6 diamidino 2 phenylindole/product/Vector Laboratories
Average 98 stars, based on 1 article reviews
4 6 diamidino 2 phenylindole - by Bioz Stars, 2026-06
98/100 stars
  Buy from Supplier
vectashield hardset antifade mounting medium with dapi  (Vector Laboratories)
96
Vector Laboratories vectashield hardset antifade mounting medium with dapi
CBA to detect anti-MuSK Abs. When HEK293 cells expressing MuSK were incubated with (A) commercially available polyclonal anti-MuSK Abs or (B) sera from patients with MuSK-Ab-positive MG, anti-IgG antibody binding (red fluorescence) was observed along the cell surface, indicating the binding of MuSK Abs to MuSK. This colocalized with GFP-labeled MuSK expressed on the surface of HEK293 cells (green fluorescence). In contrast, (C) anti-human IgG antibody binding (red fluorescence) was not observed after adding the sera from the healthy control. Scale bar: 10 µm. CBA: cell-based assay, <t>DAPI:</t> <t>4′,6-diamidino-2-phenylindole,</t> eGFP: enhanced green fluorescent protein, HEK293: human embryonic kidney 293, MG: myasthenia gravis, MuSK Abs: antibodies against muscle-specific tyrosine kinase.
Vectashield Hardset Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vectashield hardset antifade mounting medium with dapi/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
vectashield hardset antifade mounting medium with dapi - by Bioz Stars, 2026-06
96/100 stars
  Buy from Supplier

Image Search Results


CBA to detect anti-MuSK Abs. When HEK293 cells expressing MuSK were incubated with (A) commercially available polyclonal anti-MuSK Abs or (B) sera from patients with MuSK-Ab-positive MG, anti-IgG antibody binding (red fluorescence) was observed along the cell surface, indicating the binding of MuSK Abs to MuSK. This colocalized with GFP-labeled MuSK expressed on the surface of HEK293 cells (green fluorescence). In contrast, (C) anti-human IgG antibody binding (red fluorescence) was not observed after adding the sera from the healthy control. Scale bar: 10 µm. CBA: cell-based assay, DAPI: 4′,6-diamidino-2-phenylindole, eGFP: enhanced green fluorescent protein, HEK293: human embryonic kidney 293, MG: myasthenia gravis, MuSK Abs: antibodies against muscle-specific tyrosine kinase.

Journal: Journal of Clinical Neurology (Seoul, Korea)

Article Title: Evaluating an In-House Cell-Based Assay for Detecting Antibodies Against Muscle-Specific Tyrosine Kinase in Myasthenia Gravis

doi: 10.3988/jcn.2021.17.3.400

Figure Lengend Snippet: CBA to detect anti-MuSK Abs. When HEK293 cells expressing MuSK were incubated with (A) commercially available polyclonal anti-MuSK Abs or (B) sera from patients with MuSK-Ab-positive MG, anti-IgG antibody binding (red fluorescence) was observed along the cell surface, indicating the binding of MuSK Abs to MuSK. This colocalized with GFP-labeled MuSK expressed on the surface of HEK293 cells (green fluorescence). In contrast, (C) anti-human IgG antibody binding (red fluorescence) was not observed after adding the sera from the healthy control. Scale bar: 10 µm. CBA: cell-based assay, DAPI: 4′,6-diamidino-2-phenylindole, eGFP: enhanced green fluorescent protein, HEK293: human embryonic kidney 293, MG: myasthenia gravis, MuSK Abs: antibodies against muscle-specific tyrosine kinase.

Article Snippet: After washing three times with PBS, the coverslips were gently removed from the 24-well plates and placed on microscope slides with VECTASHIELD antifade mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing, Incubation, Binding Assay, Fluorescence, Labeling, Cell Based Assay